Cyclic AMP induces metallothionein gene expression in rat hepatocytes but not in rat kidney
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V L Nebes1, D DeFranco, S M Morris
1Department of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh, PA 15261.
The Biochemical Journal
|October 15, 1988
Abstract:
Cyclic AMP analogues induced expression of metallothionein-I (MT-I) mRNA in adult rat liver and in rat hepatocytes cultured in serum-free medium. This induction occurred via an increased rate of transcription of the MT-I gene. The effect of cyclic AMP analogues was tissue-specific since no induction of MT-I mRNA occurred in rat kidney. Induction of MT-I mRNA by a combination of cyclic AMP analogue and dexamethasone was additive in liver and cultured hepatocytes, indicating that induction occurred via independent regulatory pathways.