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MicroRNA-195 inhibits human gastric cancer by directly targeting basic fibroblast growth factor

J Wang1,2, L Li2, M Jiang2

  • 1Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230032, Anhui, China.

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
|May 14, 2017
PubMed
Abstract

Insights

MicroRNA-195-5p suppresses gastric cancer (GC) development by targeting basic fibroblast growth factor (bFGF). This finding highlights miR-195-5p and bFGF as potential therapeutic targets for GC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GC) is a leading cause of cancer mortality globally.
  • Basic fibroblast growth factor (bFGF) is implicated in GC progression, but its regulatory mechanisms are not fully understood.

Purpose of the Study:

  • To investigate the role of miR-195-5p in gastric cancer.
  • To elucidate the relationship between miR-195-5p and bFGF in GC development.

Main Methods:

  • Expression analysis of miR-195-5p and bFGF using real-time PCR and western blot.
  • Luciferase activity assays to confirm direct targeting of bFGF by miR-195-5p.
  • In vitro (cell migration and invasion assays) and in vivo (xenograft mouse model) experiments to assess the functional impact of miR-195-5p and bFGF.

Main Results:

  • A negative correlation was observed between miR-195-5p and bFGF expression in human GC tissues.
  • MiR-195-5p directly targets the 3'-UTR of bFGF, leading to its downregulation.
  • Downregulation of bFGF by miR-195-5p inhibited GC cell migration, invasion, and tumorigenesis in vivo, effects that were reversible upon bFGF reintroduction.

Conclusions:

  • MiR-195-5p exerts tumor-suppressive effects in gastric cancer by inhibiting bFGF.
  • Both miR-195-5p and bFGF represent promising therapeutic targets for gastric cancer treatment.
Keywords:
Gastric cancerInvasionbFGFmiR-195-5pmiRNAs

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