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CRISPR/Cas system for yeast genome engineering: advances and applications
Vratislav Stovicek1, Carina Holkenbrink1, Irina Borodina1
1The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
FEMS Yeast Research
|May 16, 2017
Summary
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) technology offers powerful genome editing and transcriptional regulation in yeast. This review covers CRISPR principles, design, and diverse applications across various yeast species.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system has revolutionized genome engineering.
- Yeast species are crucial model organisms and biotechnological tools.
- CRISPR applications in yeast are rapidly expanding.
Purpose of the Study:
- To provide a comprehensive review of CRISPR/Cas system applications in yeast.
- To cover fundamental principles, genetic design strategies, and diverse uses of CRISPR in yeast.
- To consolidate current knowledge for researchers and practitioners.
Main Methods:
- Literature review of CRISPR/Cas system applications in various yeast species.
- Analysis of genetic design principles for CRISPR tools in yeast.
- Compilation of reported applications in genome editing and transcriptional regulation.
Main Results:
- CRISPR/Cas systems are versatile tools for precise genome editing in yeast.
- These systems enable sophisticated transcriptional regulation strategies in diverse yeast models.
- A wide range of applications are demonstrated across different yeast species.
Conclusions:
- CRISPR/Cas technology is a powerful and adaptable platform for yeast research and biotechnology.
- Further development of CRISPR tools will enhance capabilities in yeast genetic manipulation.
- This review serves as a valuable resource for leveraging CRISPR in yeast systems.
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