Engineering the yeast Yarrowia lipolytica for biomanufacturing

Area of Science:

• Industrial Biotechnology
• Microbial Engineering
• Synthetic Biology

Background:

• Yarrowia lipolytica is a key oleaginous yeast with robust physiology and metabolic versatility.
• It is increasingly utilized as a cell factory in industrial biotechnology.

Purpose of the Study:

• To review recent advancements in the metabolic engineering of Yarrowia lipolytica.
• To highlight progress in genetic editing, systems-level approaches, and biomanufacturing applications.

Main Methods:

• Review of genetic engineering toolkits, including CRISPR editing and high-throughput genome engineering.
• Application of genome-scale metabolic modeling and omics data.
• Systems metabolic engineering strategies.

Main Results:

• Significant progress in developing Y. lipolytica strains through advanced genetic tools.
• Integration of systems-level approaches accelerates strain development.
• Demonstrated potential for various industrial and biotechnological applications.

Conclusions:

• Yarrowia lipolytica is a leading microbial platform for biomanufacturing.
• Continued advancements position it as a versatile workhorse for sustainable production.