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Development and Characterization of an Infectious cDNA Clone of Equine Arteritis Virus
Udeni B R Balasuriya1, Jianqiang Zhang2
1Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546, USA. ubalasuriya@uky.edu.
Abstract:
Development and characterization of several infectious cDNA clones of equine arteritis virus (EAV) have been described in the literature. Here we describe the assembly of the full-length infectious cDNA clone of the virulent Bucyrus strain (VBS; ATCC VR-796) of EAV in a plasmid vector. This system allows generation of infectious in vitro-transcribed (IVT) RNA from the linearized plasmid that can be transfected or electroporated into mammalian cells to produce infectious recombinant progeny virus. This is an efficient reverse genetics system that allows easy manipulation of EAV genomes to study molecular biology of the virus and pathogenesis of equine viral arteritis.
