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Updated: Mar 2, 2026
11:31
Generation of Recombinant Influenza Virus from Plasmid DNA
Published on: August 3, 2010
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Reverse Genetics of Influenza B Viruses
Aitor Nogales1, Daniel R Perez2, Jefferson Santos2
1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY, 14642, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 17, 2017
Summary
Reverse genetics enables the creation of influenza B viruses from plasmid DNA, advancing research into viral biology and vaccine development. This powerful technique allows genetic engineering for studying influenza pathogenesis and antiviral strategies.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Influenza B viruses contribute to annual epidemics.
- Plasmid-based reverse genetics, initially for influenza A, allows generating viruses from cDNA.
- This technology facilitates studying viral replication, host factors, and pathogenesis.
Purpose of the Study:
- To describe the cloning of influenza B viral RNAs into a plasmid.
- To detail the experimental procedures for generating recombinant influenza B viruses.
Main Methods:
- Utilized plasmid-based reverse genetics.
- Cloned influenza B/Brisbane/60/2008 viral RNAs into the ambisense pDP-2002 plasmid.
- Performed experimental procedures for recombinant virus generation.
Main Results:
- Successfully generated recombinant influenza B viruses entirely from plasmid DNA.
- Established a method for reverse genetics of influenza B viruses.
Conclusions:
- Reverse genetics is a valuable tool for influenza B virus research.
- This technique aids in understanding influenza B virus biology, pathogenesis, and vaccine development.
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