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Related Concept Videos

Thermosensation01:43

Thermosensation

34.1K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
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Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

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The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
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G-protein Coupled Receptors01:21

G-protein Coupled Receptors

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G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
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Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

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Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
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Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

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G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
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GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

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Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
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Related Experiment Video

Updated: Mar 2, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

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TRPC Channel Structure and Properties.

Shengjie Feng1

  • 1Department of Physiology, University of California, San Francisco, CA, USA. sjfeng100@gmail.com.

Advances in Experimental Medicine and Biology
|May 17, 2017
PubMed
Summary
This summary is machine-generated.

Transient Receptor Potential Canonical (TRPC) channels regulate intracellular calcium. Their structures remain unknown due to complex challenges, necessitating further research and tool development for TRPC channel analysis.

Keywords:
PropertyStructureTRPC

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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Area of Science:

  • Molecular Biology
  • Cell Physiology
  • Biophysics

Background:

  • Transient Receptor Potential Canonical (TRPC) channels are key regulators of intracellular calcium (Ca2+).
  • These nonselective cation channels play crucial roles in various physiological and pathological processes.
  • TRPC channels assemble as homo- or heterotetramers, responding to diverse stimuli.

Purpose of the Study:

  • To review the known properties and functional domains of TRPC channels.
  • To highlight the challenges hindering the structural elucidation of TRPC channels.
  • To emphasize the need for advanced tools for TRPC channel research.

Main Methods:

  • Electrophysiological techniques to study channel function.
  • Biochemical assays to identify protein interactions and domains.
  • Structural biology approaches (though limited by sample properties).

Main Results:

  • TRPC channels' roles in Ca2+ homeostasis are well-established.
  • Functional domains and properties have been characterized.
  • Significant hurdles exist in determining TRPC channel structures due to their size, instability, and flexibility.

Conclusions:

  • Understanding TRPC channel structure is critical for elucidating their function.
  • Current methods are insufficient for resolving TRPC channel structures.
  • Development of specific antibodies, agonists, and antagonists is essential for future TRPC research.