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Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics.

Stephen A Schendel1

  • 1Stanford University Medical Center, Stanford, CA.

Aesthetic Surgery Journal
|May 17, 2017
PubMed
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Autologous collagen matrix derived from liposuctioned fat provides a viable source for soft tissue fillers and stem cell therapies. This processed fat yields collagen and viable cells, offering potential for long-term regenerative results.

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Autologous collagen is a promising soft tissue filler and a matrix for stem cell applications.
  • Limited availability of autologous collagen sources has hindered its widespread use.
  • Liposuction, a common procedure, presents a potential source for autologous collagen procurement.

Purpose of the Study:

  • To characterize the collagen content and composition of adipose-derived tissue.
  • To evaluate an adipose-derived tissue product created using ultrasonic cavitation and cryo-grinding.
  • To assess the cellular and protein composition of the final collagen matrix product.

Main Methods:

  • Adipose tissue was obtained from patients undergoing liposuction.

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  • A two-step process involving ultrasonic cavitation and cryo-grinding was used to isolate connective tissue.
  • The resulting collagen matrix was analyzed using scanning electron microscopy, Western blot, and flow cytometry.

    • Main Results:

    • An average of 1 mL of collagen matrix was obtained per 100 mL of liposuctioned fat.
    • Significant viable cell markers were identified, including adipocytes, CD90+, and CD105+.
    • Western blot analysis confirmed the presence of collagen types II, III, and IV, while electron microscopy revealed a cross-linked helical collagen structure.

    Conclusions:

    • Liposuctioned fat can be processed to yield a collagen matrix with viable cells using ultrasonic separation.
    • The process preserves the overall cellular composition of the tissue.
    • This autologous collagen matrix holds potential for soft tissue restoration and regenerative therapies with long-term benefits.