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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Related Experiment Video

Updated: Mar 2, 2026

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Published on: February 9, 2020

Lymph node effective vascular permeability and chemotherapy uptake.

Eelco F J Meijer^1,2, Cedric Blatter^2,3, Ivy X Chen^1,2

¹Edwin L. Steele Laboratories for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Boston, MA, USA.

Microcirculation (New York, N.Y. : 1994)

|May 17, 2017

Summary

This summary is machine-generated.

Vascular permeability in lymph nodes can be increased by vascular endothelial growth factor-A (VEGF-A). However, this did not improve chemotherapy drug penetration into lymph node metastases.

Keywords:

chemotherapy drug penetration intravital microscopy lymph node vascular permeability

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Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

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Last Updated: Mar 2, 2026

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

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Published on: February 9, 2020

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Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

Published on: June 26, 2019

Area of Science:

Oncology
Vascular Biology
Pharmacology

Background:

Lymph node metastases are a significant prognostic factor in cancer.
Therapeutic responses in lymph node metastases can differ from primary tumors.
Understanding lymph node vasculature is crucial for optimizing systemic drug delivery.

Purpose of the Study:

To measure effective vascular permeability in lymph node blood vessels.
To investigate if increasing vascular permeability enhances chemotherapy penetration into lymph nodes.

Main Methods:

Developed a novel 3D method to measure in vivo effective vascular permeability in murine lymph nodes.
Administered vascular endothelial growth factor-A (VEGF-A) to increase vascular permeability.
Quantified chemotherapeutic drug concentrations using HPLC in various tissues.

Main Results:

VEGF-A significantly increased lymph node blood vessel effective vascular permeability (approximately fourfold).
No significant difference in lymph node drug accumulation was observed between VEGF-A treated and untreated groups.
Chemotherapy penetration into lymph nodes was not enhanced by VEGF-A pretreatment.

Conclusions:

Lymph node vascular permeability is modifiable by VEGF-A.
Increasing vascular permeability alone does not guarantee improved chemotherapy uptake in lymph nodes.
Further strategies are needed to enhance drug delivery to lymph node metastases.