Related Experiment Video For Cervical malignancy
Updated: Mar 2, 2026
An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
Published on: May 13, 2019
Micronucleus Assay in Urothelial Cells in Cancer Cervix
Suresh Kumar Sundararajan1, Pratheepa Sivasankari Natarajan2, Kanchana3
1Assistant Professor, Department of Medical Oncology, Madras Medical College and Hospital, Chennai, Tamil Nadu, India.
Introduction:
Cancer ranks third among the ten leading global causes of death. To evaluate the genotoxic risks, observed as DNA damages, can be assessed by Micronucleus (MN) test.
Aim:
To identify the occurrence of MN in normal and cancer cervix and find the correlation between MN and stage of cancer.
Materials And Methods:
A total of 60 females were included in the study and visual examination of the cervix was done. Based on the examination two groups were formed: A- Normal cervix (n-23) and B- Presence of erosion or growth or ulcer etc., in cervix (n-37). Midstream urine sample was collected and centrifuged from the cases after getting the informed consent. Slides were prepared from the pellet, were fixed in methanol, glacial acetic acid fixative and stained with Giemsa and May Grunwald stain. Statistical analysis was done by student's t-test and chi-square test.
Results:
A linear association was noted between the mean MN count and cancer cervix stage. Almost 18.2% of the Group A cases had significant MN count. Sensitivity and specificity of MN count in Group A was 83.8% and 82.6% respectively. The efficiency was 83.3%.
Conclusion:
A statistically significant MN count was seen in the different stages of cancer cervix. There are cases who had normal findings on visual inspection of cervix but with significant MN count are prone for malignant transformation. MN assay is an easy, non-invasive, cost-effective method and can be used as a screening test for a large population.
