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Updated: Mar 2, 2026
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
Published on: July 29, 2018
Identification of a Human Clonogenic Progenitor with Strict Monocyte Differentiation Potential: A Counterpart of
Shunsuke Kawamura1, Nobuyuki Onai2, Fuyuki Miya3
1Department of Biodefense Research, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113-8510, Japan; JSPS Research Fellow of Japan Society for Promotion of Science (JSPS), Tokyo 102-0083, Japan.
Abstract:
Monocytes give rise to macrophages and dendritic cells (DCs) under steady-state and inflammatory conditions, thereby contributing to host defense and tissue pathology. A common monocyte progenitor (cMoP) that is strictly committed to the monocyte lineage has been recently identified in mice. Here, we identified human cMoPs as a CLEC12AhiCD64hi subpopulation of conventional granulocyte-monocyte progenitors (cGMPs) in umbilical cord blood and in bone marrow. Human cMoPs gave rise to monocyte subsets without showing any potential for differentiating into myeloid or lymphoid cells. Within the cGMP population, we also identified revised GMPs that completely lacked DC and lymphoid potential. Collectively, our findings expand and revise the current understanding of human myeloid cell differentiation pathways.
Insights
Researchers identified human common monocyte progenitors (cMoPs) as a specific cell population. These progenitors exclusively generate monocytes, refining our understanding of human myeloid cell development.
Area of Science:
- Immunology
- Hematopoiesis
- Cell Biology
Background:
- Monocytes are crucial immune cells differentiating into macrophages and dendritic cells (DCs).
- Understanding monocyte progenitor development is key to comprehending immune responses and pathologies.
- Previous studies identified a common monocyte progenitor (cMoP) in mice.
Purpose of the Study:
- To identify and characterize human common monocyte progenitors (cMoPs).
- To define the differentiation potential of identified human progenitor populations.
- To revise the current model of human myeloid cell differentiation.
Main Methods:
- Flow cytometry was used to identify specific cell surface markers (CLEC12A, CD64) on progenitor cells.
- Functional assays were performed to assess the differentiation capacity of identified progenitor subsets.
- Analyses were conducted on human umbilical cord blood and bone marrow samples.
Main Results:
- Human cMoPs were identified as a CLEC12A-high, CD64-high subpopulation within conventional granulocyte-monocyte progenitors (cGMPs).
- These human cMoPs demonstrated a strict commitment to monocyte lineage, generating monocyte subsets only.
- Revised GMPs within the cGMP population were identified, lacking DC and lymphoid differentiation potential.
Conclusions:
- The study successfully identified and characterized human common monocyte progenitors (cMoPs).
- These findings refine the understanding of human myeloid progenitor hierarchy and lineage commitment.
- The results expand and revise the current model of human myeloid cell differentiation pathways.
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