Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 2, 2026

Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

16.7K

Sample Preparation for Mass Cytometry Analysis.

Ryan L McCarthy1, Aundrietta D Duncan2, Michelle C Barton2

  • 1Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center; RLMcCarthy@mdanderson.org.

Journal of Visualized Experiments : Jove
|May 19, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developing a Clinically Practical Biomaterial Platform for Endogenous Liver Regeneration.

Gels (Basel, Switzerland)·2026
Same author

A Linkable, Polycarbonate Gut Microbiome-Distal Tumor Chip Platform for Interrogating Cancer Promoting Mechanisms.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024
Same author

ERH Enables Early Embryonic Differentiation and Overlays H3K9me3 Heterochromatin on a Cryptic Pluripotency H3K9me3 Landscape in Somatic Cells.

bioRxiv : the preprint server for biology·2024
Same author

Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24.

iScience·2023
Same author

Diverse heterochromatin states restricting cell identity and reprogramming.

Trends in biochemical sciences·2023
Same author

A pioneer factor locally opens compacted chromatin to enable targeted ATP-dependent nucleosome remodeling.

Nature structural & molecular biology·2022
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

This study presents an optimized protocol for mass cytometry, enhancing data quality and minimizing variability. Following these methods ensures reliable, high-quality results for deep biological analysis.

Area of Science:

  • Biotechnology
  • Immunology
  • Cellular Biology

Background:

  • Conventional fluorescence-based flow cytometry has limitations in parameter detection.
  • Mass cytometry offers increased analytical depth through heavy metal-conjugated antibodies.
  • Ensuring data reliability in novel techniques like mass cytometry is crucial.

Purpose of the Study:

  • To provide an optimized protocol for cell sample processing in mass cytometry.
  • To guide experimental design by explaining optional steps and their biological implications.
  • To ensure consistent, high-quality data generation and minimize variability.

Main Methods:

  • Detailed protocol for cell sample preparation for mass cytometry.
  • Incorporation of multiple techniques to optimize data quality.

More Related Videos

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
08:09

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Published on: June 19, 2019

10.0K
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

10.0K

Related Experiment Videos

Last Updated: Mar 2, 2026

Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

16.7K
Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
08:09

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Published on: June 19, 2019

10.0K
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

10.0K
  • Explanation of optional steps for tailored experimental design.

    • Main Results:

    • Minimized variability leading to consistent and reliable mass cytometry data.
    • Avoidance of common pitfalls in cell sample processing.
    • Representative data illustrating expected outcomes from the optimized protocol.

    Conclusions:

    • The presented protocol enhances the reliability and quality of mass cytometry data.
    • Optimized sample processing is key to accurate deep biological analysis.
    • This protocol supports researchers in achieving consistent results and uncovering new biological insights.