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Related Concept Videos

Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

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A Linkable, Polycarbonate Gut Microbiome-Distal Tumor Chip Platform for Interrogating Cancer Promoting Mechanisms.

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Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 18, 2024
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Summary

A novel organ-on-chip platform made from polycarbonate enables the study of gut microbiome interactions with human gut tissues. This technology overcomes limitations of previous models, facilitating research into gut bacteria and disease mechanisms.

Keywords:
cancergut microbiomesmicrophysiological systemsorgan‐on‐chips

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Area of Science:

  • Biomedical Engineering
  • Microbiology
  • Gastroenterology

Background:

  • Gut microbiome composition is linked to various diseases, but underlying mechanisms remain unclear, hindering therapeutic development.
  • Organ-on-chip technology offers a controlled environment to study tissue and microbial interactions.
  • Existing gut-on-chip models often use oxygen-permeable materials, complicating the study of anaerobic gut bacteria.

Purpose of the Study:

  • To develop an improved organ-on-chip platform for studying gut microbiome-host interactions.
  • To overcome material limitations in current organ-on-chip devices for anaerobic bacterial co-culture.
  • To enable high-resolution imaging and biomolecule studies of gut microbiota-epithelia communication.

Main Methods:

  • Fabrication of organ-on-chip devices entirely from gas-impermeable polycarbonate.
  • Replication of polarized villus-like structures within the polycarbonate chips.
  • Co-culture of anaerobic bacteria (Blautia coccoides) with gut epithelia on-chip.
  • Development of a secondary chip design for hydrogel-embedded tissue culture and on-chip processing.

Main Results:

  • The polycarbonate chips successfully supported co-culture of anaerobic bacteria with gut epithelia for two days in a standard incubator.
  • The gas-impermeable nature of the chips eliminated the need for interventions to maintain anaerobic conditions.
  • The platform demonstrated suitability for high-resolution microscopy and on-chip processing, addressing material absorption issues.

Conclusions:

  • The developed polycarbonate organ-on-chip platform provides a robust system for studying anaerobic gut bacteria and host interactions.
  • This technology facilitates mechanistic studies of the gut microbiome's role in health and disease.
  • The platform's design enables future investigations into microbiota-host communication, including interactions with distal tissues like tumors.