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Analyzing and Building Nucleic Acid Structures with 3DNA
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Core-Shell and Layer-by-Layer Assembly of 3D DNA Crystals.

Ronald McNeil1, Paul J Paukstelis1

  • 1Chemistry & Biochemistry Department, Center for Biomolecular Structure and Organization, Maryland NanoCenter, University of Maryland, College Park, MD, 20742, USA.

Advanced Materials (Deerfield Beach, Fla.)
|May 19, 2017
PubMed
Summary

Researchers created layered DNA crystals for advanced molecular scaffolds. This breakthrough enables the development of novel biomaterials with tunable properties for diverse applications.

Keywords:
DNA crystalsDNA nanotechnologybiomaterialslayer-by-layer assembly

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Area of Science:

  • DNA nanotechnology
  • Materials science
  • Crystallography

Background:

  • DNA nanotechnology aims to create 3D crystals for molecular scaffolding.
  • The BET66 DNA 13-mer self-assembles into crystals using various base-pairing rules.
  • These DNA crystals feature solvent channels capable of holding guest molecules.

Purpose of the Study:

  • To demonstrate core-shell crystal growth in DNA nanotechnology.
  • To create multilayered DNA crystals with distinct, layer-specific functionalities.
  • To explore the potential of DNA crystals as tunable, periodic biomaterials.

Main Methods:

  • Self-assembly of the BET66 DNA 13-mer into crystalline structures.
  • Incorporation of guest molecules during crystal assembly to form distinct layers.
  • Post-crystallization covalent attachment of guest molecules within crystal channels.

Main Results:

  • Successful assembly of DNA crystals with two or more discrete layers (core-shell growth).
  • Layers are structurally identical at the DNA level but differentiated by guest molecule presence.
  • Demonstrated layer-specific covalent attachment of guest molecules via crystal solvent channels.

Conclusions:

  • This study presents the first example of biomacromolecular core-shell crystal growth using DNA.
  • Controlling guest molecule properties allows for the creation of multifunctional periodic biomaterials.
  • This approach offers a new route to engineer DNA-based materials with tailored optical, chemical, and physical characteristics.