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Related Experiment Video

Updated: Mar 2, 2026

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Published on: November 29, 2012

Imaging membrane potential changes from dendritic spines using computer-generated holography.

Dimitrii Tanese¹, Ju-Yun Weng², Valeria Zampini¹

¹Paris Descartes University, Neurophotonics Laboratory, CNRS UMR8250, Paris, France.

|May 20, 2017

Summary

This summary is machine-generated.

Voltage imaging now allows detailed measurement of neuronal electrical activity. Combining this with patterned illumination and glutamate uncaging minimizes photodynamic damage and signal contamination, enabling parallel spine imaging.

Keywords:

dendritic spines glutamate uncaging holography voltage imaging

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Published on: May 4, 2014

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons

Published on: July 13, 2011

Related Experiment Videos

Last Updated: Mar 2, 2026

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Published on: November 29, 2012

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Published on: May 4, 2014

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons

Published on: July 13, 2011

Area of Science:

Neuroscience
Biophysics
Optical Imaging

Background:

Neuronal electrical properties are complex and difficult to measure.
Subthreshold electrical events in dendrites are crucial for action potential initiation.
Voltage imaging offers a way to measure electrical signals at the scale of dendritic spines.

Purpose of the Study:

To combine voltage imaging with glutamate uncaging using computer-generated holography.
To investigate the benefits of patterned illumination for reducing photodynamic damage and optical signal contamination.
To enable parallel imaging of multiple dendritic spines.

Main Methods:

Voltage imaging with voltage-sensitive probes.
Glutamate uncaging using computer-generated holography.
Patterned illumination techniques.

Main Results:

Patterned illumination reduced photodynamic damage by limiting the illuminated membrane area.
Region-specific illumination minimized optical signal contamination from scattered light.
Parallel one-photon glutamate uncaging was achieved alongside voltage imaging.

Conclusions:

Combined voltage imaging and patterned illumination offer a powerful method for studying neuronal electrical activity.
This technique minimizes experimental artifacts, improving signal quality and enabling parallel measurements.
The approach facilitates detailed investigation of synaptic integration and action potential initiation at the single-spine level.