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Related Experiment Video

Updated: Mar 2, 2026

The Use of an Automated System GreenFeed to Monitor Enteric Methane and Carbon Dioxide Emissions from Ruminant Animals

The Use of an Automated System GreenFeed to Monitor Enteric Methane and Carbon Dioxide Emissions from Ruminant Animals

Published on: September 7, 2015

Development of Multiwell-Plate Methods Using Pure Cultures of Methanogens To Identify New Inhibitors for Suppressing

M R Weimar¹, J Cheung¹, D Dey²

¹Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.

Applied and Environmental Microbiology

|May 21, 2017

Summary

This summary is machine-generated.

Researchers developed a high-throughput 96-well plate method for growing methanogens, enabling faster screening of compounds to find inhibitors for reducing ruminant methane emissions.

Keywords:

Methanobrevibacter Methanococcus maripaludis greenhouse gas high-throughput methanogen rumen

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Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Published on: January 31, 2025

Area of Science:

Microbiology
Environmental Science
Biotechnology

Background:

Hydrogenotrophic methanogens are crucial in ruminant digestion but require difficult, low-throughput anaerobic culturing.
Methane emissions from ruminants significantly contribute to global greenhouse gas emissions, necessitating novel mitigation strategies.
Current methods for identifying methanogen inhibitors are hindered by the lack of high-throughput growth assays.

Purpose of the Study:

To develop and optimize a high-throughput 96-well microtiter plate assay for culturing methanogens.
To validate the assay's robustness, reproducibility, and sensitivity for screening chemical libraries.
To identify novel methanogen-specific inhibitors for mitigating ruminant methane emissions.

Main Methods:

Optimized key parameters including inoculum size, reducing agents, assay duration, solvent compatibility, and culture volume for 96-well plates.
Validated the assay using known methanogen inhibitors and statistical analysis.
Screened Sigma-Aldrich LOPAC and an in-house natural product library against *Methanococcus maripaludis*.

Main Results:

Successfully developed and optimized a high-throughput 96-well microtiter plate method for growing marine and rumen methanogens.
Validated the assay's sensitivity and reproducibility, identifying bioactive compounds from compound libraries.
Confirmed minimum inhibitory concentration (MIC) values for identified inhibitors against *M. maripaludis* and *Methanobrevibacter* species AbM4.

Conclusions:

The developed high-throughput assay significantly increases the capacity for screening compound libraries against methanogens.
This platform facilitates the discovery of novel methanogen inhibitors, crucial for developing agritech solutions to reduce ruminant methane emissions.
The method provides a powerful tool for accelerating research into controlling greenhouse gas emissions from livestock agriculture.