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Single-step PCR-based genetic sex determination of rat tissues and cells

Pramod Dhakal1, Michael J Soares1

  • 1Institute for Reproductive Health and Regenerative Medicine, Department of Pathology & Laboratory Medicine and Department of Pediatrics, University of Kansas Medical Center, Kansas City, KS.

Biotechniques
|May 23, 2017
PubMed

Keywords:
fetalratsex determination

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In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.  
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