Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

4.1K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
4.1K
Enteric Nervous System: Regulation of GI Motor Activity01:11

Enteric Nervous System: Regulation of GI Motor Activity

2.0K
The Enteric Nervous System (ENS) plays a pivotal role in regulating gastrointestinal or GI motor activity. This complex network of nerves, deeply embedded within the gut wall, responds to changes in the gut environment and receives input from both the autonomic nervous system and the central nervous system. By doing so, the ENS operates various programs tailored to the body's nutritional status and needs.
During periods of fasting, the ENS initiates the migrating myoelectric complex, a...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional maturation of enteric neurons derived from human induced pluripotent stem cells.

Stem cell reports·2026
Same author

Factors to Consider in Gut Microbiota Interactions With the Enteric Nervous System-What You Look for Is What You Get.

Cellular and molecular gastroenterology and hepatology·2026
Same author

Strengthening the Future of Nursing Education.

Research in gerontological nursing·2026
Same author

Caregivers with poorer health are less likely to engage in an intervention to prepare for their own health care emergency.

The Gerontologist·2026
Same author

Rural Aging and Health Equity.

Research in gerontological nursing·2026
Same author

Functional characterization and classification of enteric neurons, and regional differences in neural control of digestive functions.

Autonomic neuroscience : basic & clinical·2026
Same journal

The molecular mechanism underlying melatonin-mediated repair of ovarian damage in mice exposed to abnormal light cycles.

Developmental biology·2026
Same journal

The Drosophila ovarian terminal filament imports lipophilic molecules that support cyst and follicle development within the ovariole.

Developmental biology·2026
Same journal

Secreted Frizzled-Related Protein 1 Controls Distal Lung Formation via Wnt and PDGF Signaling.

Developmental biology·2026
Same journal

The vascular-osteogenic interface in craniofacial development: a structured review of emerging associations in congenital malformations.

Developmental biology·2026
Same journal

Turning off metamorphosis: Thyroid hormone deregulation in the evolution of obligately paedomorphic salamanders.

Developmental biology·2026
Same journal

Developmental analysis of the cone photoreceptor-less little skate retina reveals distinct Onecut1 isoforms.

Developmental biology·2026
See all related articles

Related Experiment Video

Updated: Mar 2, 2026

In Situ Ca2+ Imaging of the Enteric Nervous System
11:26

In Situ Ca2+ Imaging of the Enteric Nervous System

Published on: January 29, 2015

18.0K

Spontaneous calcium waves in the developing enteric nervous system.

Marlene M Hao1, Annette J Bergner2, Caroline S Hirst2

  • 1Department of Anatomy and Neuroscience, University of Melbourne, Australia; Laboratory for Enteric Neuroscience, TARGID, University of Leuven, Belgium.

Developmental Biology
|May 23, 2017
PubMed
Summary
This summary is machine-generated.

Enteric neural crest-derived cells (ENCCs) communicate via calcium waves, not gap junctions. Purinergic signaling through P2 receptors is crucial for ENCC network formation during embryonic development.

Keywords:
Calcium imagingEnteric nervous systemNeural crest cellsParacrine communicationPurinergic signallingSpontaneous calcium wave

More Related Videos

Functional Calcium Imaging in Developing Cortical Networks
16:33

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

39.7K
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

8.6K

Related Experiment Videos

Last Updated: Mar 2, 2026

In Situ Ca2+ Imaging of the Enteric Nervous System
11:26

In Situ Ca2+ Imaging of the Enteric Nervous System

Published on: January 29, 2015

18.0K
Functional Calcium Imaging in Developing Cortical Networks
16:33

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

39.7K
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

8.6K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • The enteric nervous system (ENS) originates from neural crest-derived cells (ENCCs).
  • Communication between ENCCs is essential for ENS development but poorly understood.
  • Neural crest cells influence each other's behavior, necessitating intercellular communication.

Purpose of the Study:

  • To investigate the mechanisms of communication between ENCCs in the embryonic gut.
  • To identify the signaling pathways involved in ENCC communication.
  • To understand the role of intercellular communication in ENCC network formation.

Main Methods:

  • Utilized Ca2+ imaging in mice engineered to express a genetically-encoded calcium indicator in ENCCs.
  • Performed pharmacological experiments to test wave propagation mechanisms.
  • Confirmed receptor expression using RT-PCR.

Main Results:

  • Observed spontaneous, propagating calcium waves between neighboring ENCCs.
  • Demonstrated that wave propagation relies on purinergic signaling via P2 receptors, not gap junctions.
  • Showed that P2 receptor inhibition alters ENCC network morphology without affecting differentiation or proliferation.

Conclusions:

  • Purinergic signaling mediates Ca2+ wave propagation between ENCCs during prenatal development.
  • This purinergic signaling is critical for the formation of the ENCC network.
  • Calcium waves likely play a significant role in enteric ganglia development, similar to their role in sympathetic ganglia formation.