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$Interference and Diffraction$ 02:18

Interference and Diffraction

Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.

Atomic Absorption Spectroscopy: Interference

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...

Atomic Emission Spectroscopy: Interference

Atomic Emission Spectroscopy: Interference

In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...

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Related Experiment Video

Updated: Mar 2, 2026

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

Published on: August 12, 2013

An experimental check of backscattering interferometry.

Michael M Baksh¹, M G Finn¹

¹School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30306, USA.

Sensors and Actuators. B, Chemical

|May 23, 2017

Summary

This summary is machine-generated.

Backscattering interferometry (BSI) accurately measured biomolecular binding constants, contradicting previous claims. This study validates BSI

Keywords:

backscattering interferometry binding constants experimental methods label-free protein-ligand interactions

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Scattering And Absorption of Light in Planetary Regoliths

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Area of Science:

Biochemistry
Analytical Chemistry
Biophysics

Background:

Backscattering interferometry (BSI) is a technique used to study biomolecular interactions.
A 2015 publication questioned the capability of BSI to measure binding constants.

Purpose of the Study:

To determine association constants for four well-established biomolecular interactions using BSI.
To address and invalidate claims from a previous study regarding BSI's measurement limitations.

Main Methods:

Utilized Backscattering Interferometry (BSI) to analyze biomolecular binding.
Investigated interactions including protein A + IgG, trypsin + antitrypsin, trypsin + p-aminobenzamidine, and antithrombin + heparin.

Main Results:

Obtained well-defined binding curves and dissociation constants (K_d) for all tested interactions.
Results showed close agreement with previously published findings obtained via alternative methods.
Directly contradicted the assertions made in a 2015 publication concerning BSI's measurement capabilities.

Conclusions:

BSI is a capable technique for determining association constants in biomolecular interactions.
The findings invalidate claims that BSI cannot perform such measurements.
Identified potential sources of error in the previously published BSI investigation.