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Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity
Juan A González-Vera1, David Bouzada, Céline Bouclier
1Institut des Biomolécules Max Mousseron, CNRS, IBMM-UMR 5247, Université de Montpellier, 15 Av. Charles Flahault, 34093 Montpellier, France. may.morris@umontpellier.fr.
Chemical Communications (Cambridge, England)
|May 23, 2017
Abstract:
We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.
