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Updated: Mar 2, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy.

E Kozma¹, G Estrada Girona², G Paci²

¹"Lendület" Chemical Biology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok krt. 2, 1117 Budapest, Hungary. kele.peter@ttk.mta.hu.

Chemical Communications (Cambridge, England)

|May 23, 2017

Summary

Researchers developed new siliconrhodamine probes for bioorthogonal tagging. These probes enable precise imaging of intracellular proteins using super-resolution microscopy.

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Last Updated: Mar 2, 2026

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Published on: December 9, 2013

Area of Science:

Chemical Biology
Molecular Imaging
Biotechnology

Background:

Genetically encoded proteins are crucial for cellular function.
Visualizing intracellular protein dynamics requires precise labeling techniques.
Super-resolution microscopy offers enhanced spatial resolution for biological imaging.

Purpose of the Study:

To synthesize novel double-fluorogenic siliconrhodamine probes.
To enable site-specific bioorthogonal tagging of intracellular proteins.
To facilitate imaging of labeled proteins using super-resolution microscopy.

Main Methods:

Synthesis of tetrazine-functionalized siliconrhodamine probes.
Application of probes for bioorthogonal labeling of genetically manipulated proteins.
Imaging of labeled proteins with super-resolution microscopy.

Main Results:

Successful synthesis of novel siliconrhodamine probes.
Demonstration of membrane permeability and site-specific labeling.
High-resolution imaging of intracellular proteins achieved.

Conclusions:

The developed probes are effective tools for intracellular protein imaging.
Bioorthogonal tagging with these probes allows precise visualization of protein localization and dynamics.
This technology advances the study of cellular processes at the molecular level.