Colorimetric detection of biothiols based on aggregation of chitosan-stabilized silver nanoparticles

We have described a simple and reliable colorimetric method for the sensing of biothiols such as cysteine, homocysteine, and glutathione in biological samples. The selective binding of chitosan capped silver nanoparticles to biothiols induced aggregation of the chitosan-Ag NPs. But the other amino acids that do not have thiol group cannot aggregate the chitosan-Ag NPs. Aggregation of chitosan-Ag NPs has been confirmed with UV-vis absorption spectra, zeta potential and transmission electron microscopy images. Under optimum conditions, good linear relationships existed between the absorption ratios (at A₅₀₀/A₄₁₅) and the concentrations of cysteine, homocysteine, and glutathione in the range of 0.1-10.0μM with detection limits of 15.0, 84.6 and 40.0nM, respectively. This probe was successfully applied to detect these biothiols in biological samples (urine and plasma).