Sensitive impedimetric DNA biosensor based on (Nb,V) codoped TiO₂ for breast cancer susceptible gene detection

In this paper, a novel (Nb,V) codoped TiO₂ (NVTO) nanoparticle was synthesized, and a novel electrochemical DNA biosensor based on NVTO was first developed for detection of breast cancer susceptible gene detection. The proposed DNA biosensors were fabricated by immobilization of the probe DNA on a nanoporous CHIT-NVTO thin film deposited on an ITO-coated glass plate. The prepared NVTO composite film was characterized by XRD, SEM and FTIR. The results indicated that the synergistic effect of CHIT-NVTO nanocomposite can increase the electroactive surface area and the loading amounts of ssDNA. These can amplify the electrochemical signal resulting in increasing the sensitivity of the proposed biosensor. The proposed biosensor exhibited high sensitivity, wide linear range of 1.0×10^-15M~1.0×10^-6M (r=0.9973) and a low detection limit of 1.09×10^-16M (S/N=3). The NVTO based biosensor opens a different perspective for susceptible gene detection and early diagnosis of breast cancer.