Prostaglandin E₂ inhibits matrix mineralization by human bone marrow stromal cell-derived osteoblasts via...

The osteoinductive properties of prostaglandin E₂ (PGE₂) and its signaling pathways have led to suggestions that it may serve as a potential therapeutic strategy for bone loss. However, the prominence of PGE₂ as an inducer of bone formation is attributed primarily to findings from studies using rodent models. In the current study, we investigated the effects of PGE₂ on human bone marrow stromal cell (hBMSC) lineage commitment and determined its mode of action. We demonstrated that PGE₂ treatment of hBMSCs significantly altered the expression profile of several genes associated with osteoblast differentiation (RUNX2 and ALP) and maturation (BGLAP and MGP). This was attributed to the activation of specific PGE₂ receptors, and was associated with increases in cAMP production and sustained AKT phosphorylation. Pharmacological inhibition of exchange protein directly activated by cAMP (Epac), but not protein kinase A (PKA), recovered the mineralization functions of hBMSC-derived osteoblasts treated with PGE₂ and restored AKT phosphorylation, along with the expression levels of RUNX2, ALP, BGLAP and MGP. Our findings therefore provide insights into how PGE₂ influences hBMSC-mediated matrix mineralization, and should be taken into account when evaluating the role of PGE₂ in human bone metabolism.