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Enzyme-Linked Immunosorbent Assay01:33

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Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
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SERS-Activated Platforms for Immunoassay: Probes, Encoding Methods, and Applications.

Zhuyuan Wang1, Shenfei Zong1, Lei Wu1

  • 1Advanced Photonics Center, Southeast University , Nanjing 210096, Jiangsu, China.

Chemical Reviews
|May 24, 2017
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Summary
This summary is machine-generated.

Surface-enhanced Raman scattering (SERS) immunoassays offer sensitive detection for disease diagnosis and environmental monitoring. This review covers SERS immunoassay principles, platforms, and applications, while also discussing future improvements.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Nanotechnology

Background:

  • Surface-enhanced Raman scattering (SERS) immunoassays are valuable tools due to their high sensitivity, multiplexing capability, and broad dynamic range.
  • Applications span disease diagnosis, environmental monitoring, and food safety, driving innovation in assay formats.
  • Advancements include integration with automated platforms like test strips and microfluidic chips.

Purpose of the Study:

  • To provide a comprehensive overview of SERS-based immunoassays.
  • To highlight key components, including SERS nanoprobes, spectral encoding strategies, and immunoassay platforms.
  • To discuss recent biological applications and future directions for performance enhancement.

Main Methods:

  • Review of literature focusing on SERS immunoassay principles and recent advancements.
  • Analysis of typical SERS nanoprobe structures and optical spectral encoding strategies.
  • Examination of popular immunoassay platforms and their biological applications over the past five years.

Main Results:

  • SERS immunoassays demonstrate significant progress in sensitivity and application scope.
  • Key elements like nanoprobes and platforms are crucial for assay performance.
  • Representative biological applications showcase the potential of these techniques.

Conclusions:

  • SERS immunoassays are powerful for various analytical challenges, with ongoing development in automated platforms.
  • Despite advances, challenges remain in optimizing performance, necessitating further research.
  • Guidelines are provided for selecting appropriate SERS immunoassay platforms based on specific application needs.