[Immunoradiometric analysis of rat myeloperoxidase]

The immunoradiometric assay of rat myeloperoxidase (MPO) was developed based on the usage of the CNBr-activated sepharose as a solid phase for antiMPO and of [125I] IgG (the affinity preparation of antiMPO) as a label. The analysis lasted for 8 h and standard curve limits were 0.5-500 ng per tube. Intra and inter assay coefficients of variation did not exceed 3% and 6.5%, respectively. It was shown by the above method that the intensive swimming of male rats led to an increase in the MPO concentration in blood plasma and to its decrease in neutrophils.