Related Experiment Video

Updated: Mar 2, 2026

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery
08:38

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

Published on: August 16, 2019

29.7K

Abnormal expression of long non-coding RNAs in myocardial infarction

Tao Wu1, Huan-Dong Wu2, Zao-Xian Xu2

  • 1Hangzhou JunKangYiDe Hospital, No.26 North Xueyuan Road, Hangzhou, 310011, Zhejiang Province, People's Republic of China.

Heart and Vessels
|May 25, 2017
PubMed

Insights

This study identified dysregulated long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in myocardial infarction (MI) mouse models. These findings offer insights into the molecular mechanisms underlying MI pathogenesis.

Area of Science:

  • Cardiovascular Biology
  • Molecular Genetics
  • Gene Expression Profiling

Background:

  • Myocardial infarction (MI) remains a primary global cause of mortality.
  • Understanding the molecular underpinnings of MI is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate dysregulated long non-coding RNAs (lncRNAs) in myocardial infarction (MI).
  • To elucidate the underlying mechanisms of lncRNA involvement in MI pathogenesis.

Main Methods:

  • Microarray analysis of lncRNA and mRNA expression in left ventricular tissues of MI and sham mice.
  • Differential expression analysis, co-expression network construction, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) for validation of differentially expressed lncRNAs.

Main Results:

  • Identified 168 differentially expressed lncRNAs (DELs) and 126 differentially expressed mRNAs (DEMs) in MI.
  • Constructed a co-expression network involving 219 nodes and 1775 edges.
  • Validated specific lncRNAs (ENSMUST00000124047, AK166279, ENSMUST00000121611, NR_015515) with altered expression in MI.

Conclusions:

  • The study identified a comprehensive profile of dysregulated lncRNAs and mRNAs in myocardial infarction.
  • Enriched signaling pathways in MI include complement and coagulation cascades, and cytokine-cytokine receptor interactions.
  • These findings contribute to understanding MI pathogenesis and may guide future therapeutic strategies.
Keywords:
Co-expressionExpression profilingLong non-coding RNAMyocardial infarctionPathogenesis

More Related Videos

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.6K
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease04:41

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

8.7K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.8K
Myocarditis I: Introduction01:21

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...
498
Cardiomyopathy IV: Restrictive Cardiomyopathy01:29

Cardiomyopathy IV: Restrictive Cardiomyopathy

Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
658
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K