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Updated: Mar 2, 2026

Analysis of Cell Migration within a Three-dimensional Collagen Matrix
Published on: October 5, 2014
RhoA and RhoC are involved in stromal cell-derived factor-1-induced cell migration by regulating F-actin
Jixian Luo1, Dingyun Li2, Dan Wei2
1School of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, China. luojx@sxu.edu.cn.
Abstract:
Stromal cell-derived factor-1 (SDF-1) signaling is important to the maintenance and progression of T-cell acute lymphoblastic leukemia by inducing chemotaxis migration. To identify the mechanism of SDF-1 signaling in the migration of T-ALL, Jurkat acute lymphoblastic leukemia cells were used. Results showed that SDF-1 induces Jurkat cell migration by F-actin redistribution and assembly, which is dependent on Rho activity. SDF-1 induced RhoA and RhoC activation, as well as reactive oxygen species (ROS) production, which was inhibited by Rho inhibitor. The Rho-dependent ROS production led to subsequent cytoskeleton redistribution and assembly in the process of migration. Additionally, RhoA and RhoC were involved in SDF-1-induced Jurkat cell migration. Taken together, we found a SDF-1/CXCR4-RhoA and RhoC-ROS-cytoskeleton pathway that regulates Jurkat cell migration in response to SDF-1. This work will contribute to a clearer insight into the migration mechanism of acute lymphoblastic leukemia.
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