Related Experiment Video

Updated: Mar 1, 2026

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

6.1K

MicroRNA-455 suppresses the oncogenic function of HDAC2 in human colorectal cancer

Q D Mao1, W Zhang1, K Zhao1

  • 1Department of Gastroenterology, Huangdao Division, The Affiliated Hospital of Qingdao University, Qingdao, China.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|May 25, 2017
PubMed

Insights

MicroRNA-455 targets Histone deacetylase 2 (HDAC2) to inhibit colorectal cancer (CRC) cell proliferation and induce apoptosis. This study identifies a novel therapeutic strategy for CRC by targeting the miR-455/HDAC2 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Colorectal cancer (CRC) is a major cause of cancer mortality.
  • Histone deacetylase 2 (HDAC2) plays a role in CRC prognosis and therapy.
  • Identifying novel therapeutic targets is crucial for effective CRC treatment.

Purpose of the Study:

  • To investigate the role of HDAC2 in colorectal cancer.
  • To explore microRNA-455 (miR-455) as a potential therapeutic agent targeting HDAC2 in CRC.
  • To elucidate the mechanism by which miR-455 affects CRC cell behavior.

Main Methods:

  • HDAC2 expression was quantified in CRC tissues using qRT-PCR.
  • HDAC2 knockdown was achieved via lentivirus transfection and confirmed by western blot.
  • Cell proliferation was assessed using MTT assays.
  • The interaction between miR-455 and HDAC2 was validated using luciferase assays.
  • miR-455 mimics were transfected to evaluate effects on HDAC2 expression, cell proliferation, and apoptosis (flow cytometry).

Main Results:

  • HDAC2 expression was significantly upregulated in CRC tissues.
  • HDAC2 knockdown markedly reduced CRC cell viability.
  • miR-455 was confirmed to directly target HDAC2.
  • miR-455 transfection decreased HDAC2 expression, inhibited cell proliferation, and induced significant apoptosis in CRC cells.

Conclusions:

  • miR-455 acts as a tumor suppressor in colorectal cancer by targeting HDAC2.
  • The miR-455/HDAC2 axis represents a promising therapeutic target for CRC treatment.
  • Targeting miR-455 could offer a novel strategy to inhibit CRC progression and induce cancer cell death.

More Related Videos

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.6K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

465

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.9K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.7K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
6.2K