Chlorhexidine Prevents Root Dentine Mineral Loss and Fracture Caused by Calcium Hydroxide over Time

Purpose. To evaluate the mineral ion loss of root dentine after treatment with 2% chlorhexidine solution (CHX) and to compare its yield and flexural strength (fs) after exposure to calcium hydroxide [Ca(OH)₂]. Materials and Methods. Dentine bars (DB) were made from 90 roots of bovine incisors and randomized into three groups: G_Control: distilled/deionized water (DDW), G_NaOCl: 2.5% sodium hypochlorite + 17% EDTA, and G_CHX: CHX + DDW. The release of phosphate (PO₄) and calcium (Ca) ions was measured by spectrophotometry. The DB were exposed to Ca(OH)₂ paste for 0, 30, 90, and 180 days. DB were subjected to the three-point bending test to obtain yield and fs values. The fracture patterns were evaluated (20x). Data were analyzed using Kruskal-Wallis and Dunn's post hoc tests or one- and two-way ANOVA followed by Tukey's post hoc test (α = 0.05). Results. G_CHX showed lower PO₄^3- and Ca²⁺ ionic release than G_NaOCl (p < 0.001). For yield and fs, G_CHX > G_NaOCl in all periods (p < 0.001), except for yield strength values on 90 days (p = 0.791). A larger frequency of vertical fractures was observed in G_NaOCl and that of oblique fractures in G_CHX (p < 0.05). Conclusions. CHX prevented PO₄^3- and Ca²⁺ loss and showed a tendency to preserve the yield and fs of root dentine over time following exposure to Ca(OH)₂ paste.