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RNA-seq03:21

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Next-Generation Sequencing for MicroRNA Expression Profile.

Yue Hu1, Wenjun Lan2, Daniel Miller3

  • 1College of Bioengineering, Qilu University of Technology, No. 3501, Da Xue Rd., Changqing District, Jinan, Shandong, 250353, People's Republic of China.

Methods in Molecular Biology (Clifton, N.J.)
|May 26, 2017
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) advances microRNA (miRNA) expression profiling. This review details the NGS pipeline for miRNA sequencing, from wet lab experiments to dry lab analysis.

Keywords:
454IlluminaIon TorrentMicroRNA expression profileNext-generation sequencingSOLiD

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Sequencing technologies have evolved significantly, from Sanger sequencing to Next-Generation Sequencing (NGS) and third-generation methods.
  • NGS is currently the dominant technology in the sequencing market.
  • NGS offers powerful capabilities for detailed investigation of microRNA (miRNA) expression profiles.

Purpose of the Study:

  • To provide a comprehensive overview of microRNA (miRNA) sequencing using Next-Generation Sequencing (NGS) platforms.
  • To elucidate the entire pipeline of miRNA sequencing, encompassing both experimental (wet) and computational (dry) phases.

Main Methods:

  • DNA sequencing using NGS as a foundational technique for RNA and miRNA sequencing.
  • Detailed explanation of the miRNA sequencing pipeline specific to NGS platforms.
  • Integration of wet laboratory procedures and dry laboratory bioinformatics analysis.

Main Results:

  • NGS platforms, despite variations, share core principles enabling advanced sequencing.
  • MiRNA sequencing possesses unique characteristics that are addressed within the NGS pipeline.
  • The workflow from sample preparation to data analysis for miRNA sequencing via NGS is thoroughly described.

Conclusions:

  • NGS technology is pivotal for in-depth miRNA expression profiling.
  • Understanding the complete miRNA sequencing pipeline is crucial for accurate data generation and interpretation.
  • The described methodology facilitates detailed research into miRNA biology and function.