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Updated: Aug 8, 2026

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Published on: October 15, 2014
In vitro susceptibility and resistance phenotypes in contemporary Enterobacter isolates in a university hospital in
Sofia Maraki1, Konstantinos Z Vardakas2,3, George Samonis4
1Department of Clinical Microbiology, University Hospital of Heraklion, Heraklion, Greece.
Aim:
To study the evolution in the susceptibility of Enterobacter spp. in Crete, Greece from 2010 to 2015.
Methods:
Non-duplicate isolates were studied using automated systems. Phenotypic confirmatory tests were applied.
Results:
A total of 939 Enterobacter isolates were included. Colistin was the most active antibiotic (97.9%) followed by imipenem (96.1%), gentamicin (95.7%), tigecycline (91.8%), cefepime (89.4%), chloramphenicol (85.8%), fosfomycin (85.5%), trimethoprim/sulfamethoxazole (83.3%) and piperacillin/tazobactam (73.3%). Antibiotic resistance did not increase during the study period for most antibiotics. Lower susceptibility was observed among multidrug-resistant strains and carbapenem-nonsusceptible isolates. AmpC was the most common resistant mechanism (21%); carbapenemases (3.7%) and aminoglycoside-modifying enzymes (6.5%) were also detected.
Conclusion:
A significant proportion of Enterobacter spp. was resistant to several antibiotics, most notably β-lactams.
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