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Updated: Mar 1, 2026
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
36.0K
CRISPR Editing in Biological and Biomedical Investigation
Xing-Da Ju1, Jing Xu2, Zhong Sheng Sun3,4
1School of Psychology, Northeast Normal University, Changchun, 130021, China.
Journal of Cellular Biochemistry
|May 26, 2017
Summary
The CRISPR-Cas system offers powerful genome editing for biology and medicine. While versatile, challenges like off-target effects and ethical concerns require careful consideration for future development.
Area of Science:
- Biotechnology
- Genomics
- Molecular Biology
Background:
- The CRISPR-Cas system, a prokaryotic adaptive immune mechanism, has been repurposed as a revolutionary genome editing tool.
- Ongoing advancements focus on optimizing CRISPR delivery strategies and engineering Cas proteins for enhanced functionality.
Purpose of the Study:
- To review recent developments in CRISPR systems.
- To outline the diverse applications of CRISPR in biological and biomedical research.
- To discuss current limitations and future perspectives of CRISPR technology.
Main Methods:
- Literature review of CRISPR system development and applications.
- Analysis of CRISPR's utility in fundamental and applied biological research.
- Evaluation of CRISPR's role in biomedical investigations and clinical disease treatment.
Main Results:
- CRISPR-based platforms are rapidly advancing biological and biomedical research.
- Applications span genomics, epigenomics, screening, agriculture, and disease modeling.
- Significant concerns include off-target effects and ethical considerations.
Conclusions:
- CRISPR technology presents vast potential across numerous scientific disciplines.
- Further research is needed to address limitations and ensure responsible application.
- Future development will likely expand CRISPR's capabilities while navigating ethical landscapes.
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