Biophysical and biological studies of some polymer grafted metallo-intercalators

Two water-soluble polymer-copper(II) complexes, [Cu(ip)₂(BPEI)](ClO₄)₂·H₂O (Complex 1) and [Cu(dppz)₂BPEI](ClO₄)₂·H₂O (Complex 2) with different degree of coordination have been synthesized and characterized. The interaction between the prepared complexes and CTDNA has been assessed by various physico-chemical methods The spectroscopic and the cyclic voltammetry studies have revealed that both the complexes interact with CTDNA through intercalation binding mode. Among the two complexes, Complex 2 has higher binding affinity with CTDNA. The antiproliferative activity of the complexes has been examined on human breast cancer cells, MDAMB231, adopting various techniques. The results indicate that both the polymer-copper(II) complexes are effective against the breast cancer cell line and the order of the activity is consistent with the DNA-binding ability.