Effect of vitamin D on endothelial progenitor cells function
Yoav Hammer1,2,3, Alissa Soudry1,2, Amos Levi1,2,3
1"Sackler" Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel.
Plos One
|May 26, 2017
Summary
Vitamin D supplementation improved endothelial progenitor cells (EPCs) in diabetic patients, enhancing their ability to form colonies and improving cell viability. These findings suggest a potential therapeutic role for vitamin D in managing vascular complications associated with diabetes.
Area of Science:
- Cardiovascular Research
- Endocrinology
- Cell Biology
Background:
- Endothelial progenitor cells (EPCs) are crucial for vascular repair, but their function is impaired in diabetes, contributing to cardiovascular disease.
- Calcitriol, the active form of vitamin D, shows potential cardiovascular benefits, yet its effect on EPCs is not well understood.
Purpose of the Study:
- To investigate the in vitro effects of calcitriol on EPCs from healthy individuals and patients with type 2 diabetes.
- To assess calcitriol's impact on EPC colony-forming capacity, viability, KLF-10 levels, and angiogenic markers.
Main Methods:
- EPCs were isolated from 51 type 2 diabetes patients and 23 healthy volunteers.
- Cells were cultured with or without calcitriol, and their functional capacity (CFUs, viability via MTT assay), KLF-10, and angiogenic markers were evaluated after one week.
Main Results:
- In diabetic patients, calcitriol significantly increased EPC colony formation and cell viability compared to controls.
- Calcitriol also enhanced cell viability in healthy subjects, though CFU counts and angiogenic markers showed no significant changes.
- KLF-10 levels tended to increase with calcitriol exposure in diabetic EPCs.
Conclusions:
- In vitro vitamin D (calcitriol) supplementation enhances EPC colony formation and viability in patients with diabetes mellitus.
- Further research is needed to elucidate the precise mechanisms underlying vitamin D's effects on EPCs.
- These findings suggest a potential role for vitamin D in improving endothelial function in diabetic individuals.
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