Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

18.0K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
18.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic Single-Binding Event Profiling With on-Chip Microlenses for Wash-Free Digital Biosensing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Perivascular Matrix Densification Dysregulates Angiogenesis and Activates Pro-Inflammatory Endothelial Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

DGrA: Lightweight Modulation Recognition Based on Hybrid Neural Networks.

Sensors (Basel, Switzerland)·2026
Same author

Hardware-Agnostic Imitation Learning Method for Autonomous Ultrasound Scanning Addressing Physical Deployment Discrepancies.

Sensors (Basel, Switzerland)·2026
Same author

Comprehensive analysis suggests CRIF1 is a potential target in breast cancer associated with prognosis and immune infiltration.

Annals of medicine·2026
Same author

Ginsenoside Rb1-engineered nanocomposite hydrogel promotes pressure injury repair through SIRT1-AMPK-mediated ferroptosis inhibition and angiogenesis activation.

Journal of ginseng research·2026
Same journal

Advances in application of microplasmas for non-metallic species analysis by optical spectrometry.

The Analyst·2026
Same journal

Sulfur vacancy-mediated self-photocatalysis-boosted electrochemiluminescence sensing <i>via</i> molecular oxygen activation for sensitive detection of isocarbophos.

The Analyst·2026
Same journal

Analytical challenges in mapping the subcellular metabolome and lipidome.

The Analyst·2026
Same journal

Threshold-guided multiplex PCR-LFA: a step toward UTI pathogen detection.

The Analyst·2026
Same journal

Aptamer-based CRISPR-Cas12a fluorescent biosensors for serum biomarker detection.

The Analyst·2026
Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026
See all related articles

Related Experiment Video

Updated: Mar 1, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.6K

Glass capillary based microfluidic ELISA for rapid diagnostics.

Xiaotian Tan1, Maung Kyaw Khaing Oo, Yuan Gong

  • 1Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave., Ann Arbor, MI 48109, USA. xsfan@umich.edu.

The Analyst
|May 27, 2017
PubMed
Summary
This summary is machine-generated.

A novel microfluidic Enzyme-Linked Immunosorbent Assay (ELISA) device significantly reduces assay time to 16 minutes and sample volume to 20 μL. This innovation enhances diagnostic speed and efficiency for medical and research applications.

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.4K
Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

11.1K

Related Experiment Videos

Last Updated: Mar 1, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.6K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.4K
Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

11.1K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biotechnology

Background:

  • Traditional Enzyme-Linked Immunosorbent Assay (ELISA) is vital for medical diagnostics and research but is limited by long assay times (4-6 hours) and large sample volumes (100 μL).
  • These limitations hinder rapid clinical diagnosis and real-time prognosis for rapidly progressing diseases.

Purpose of the Study:

  • To develop a user-friendly, microfluidic-based ELISA device utilizing a glass capillary array.
  • To significantly decrease assay time and required sample/reagent volumes compared to conventional plate-based ELISA methods.
  • To enhance the detection dynamic range for improved analytical sensitivity.

Main Methods:

  • Development of a microfluidic ELISA device incorporating a glass capillary array.
  • Implementation of a rapid chemiluminescent photo-imaging detection method using a commercial camera.
  • Application of a double exposure technique to increase the detection dynamic range.

Main Results:

  • The capillary-based ELISA device reduced total assay time to 16 minutes, a 5-fold decrease from traditional methods.
  • Sample and reagent volumes were decreased to 20 μL, a 5-fold reduction.
  • The double exposure method achieved a nearly 10-fold increase in the detection dynamic range compared to well-based ELISA.

Conclusions:

  • The developed microfluidic ELISA device offers a significant improvement over traditional methods in terms of speed, sample volume, and dynamic range.
  • This technology is suitable for rapid biochemical analysis in biomedicine and research laboratories.
  • The device presents a viable solution for time-sensitive diagnostic and prognostic applications.