Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

1.1K
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
1.1K
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

918
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
918
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

50.0K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
50.0K
Viruses of Archaea01:29

Viruses of Archaea

592
Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
592
DNA Microarrays02:34

DNA Microarrays

21.5K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.5K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

13.6K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Canine Fecal Microbiome Dataset: Ultra-deep Multi-platform Sequencing Across Extraction and Library Protocols.

Scientific data·2026
Same author

Characterisation of PAHs in Outdoor Air Pollution at Schools in a Medium-Sized Town, Hungary.

Toxics·2026
Same author

Conserved Kir channel mechanisms governing intrinsic excitability in human and rodent parvalbumin neurons.

Communications biology·2026
Same author

Multi-platform profiling reveals host- and cell -type-specific pseudorabies virus gene expression.

Scientific reports·2026
Same author

Veterinary Herpesviruses: Experimental Tools for Transcriptomics and Neuroscience.

Veterinary sciences·2026
Same author

<i>Cryptocalciella</i> - a new <i>Mortierellaceae</i> genus from Alpine glacier forefields.

IMA fungus·2026

Related Experiment Video

Updated: Mar 1, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

2.0K

Multi-platform analysis reveals a complex transcriptome architecture of a circovirus.

Norbert Moldován1, Zsolt Balázs1, Dóra Tombácz2

  • 1Department of Medical Biology, Faculty of Medicine, University of Szeged, Szeged, Hungary.

Virus Research
|May 28, 2017
PubMed
Summary

Researchers characterized porcine circovirus type 1 (PCV-1) transcripts using advanced sequencing, discovering nine novel RNA molecules and transcript isoforms. This work doubles the known PCV-1 transcriptome and reveals complex overlapping patterns potentially regulating gene expression.

Keywords:
Isoform sequencingLong-read sequencingPacBio sequencingPorcine circovirusShort-read sequencingTranscriptional interferenceTranscriptome

More Related Videos

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.9K
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
08:56

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

11.6K

Related Experiment Videos

Last Updated: Mar 1, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

2.0K
Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.9K
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
08:56

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

11.6K

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Porcine circovirus type 1 (PCV-1) is a significant pathogen in swine.
  • Understanding the full spectrum of PCV-1 transcripts is crucial for comprehending its replication and pathogenesis.
  • Previous studies have identified a limited number of PCV-1 transcripts.

Purpose of the Study:

  • To comprehensively characterize the transcriptome of porcine circovirus type 1 (PCV-1).
  • To identify novel RNA molecules and transcript isoforms with single base-pair accuracy.
  • To investigate transcript overlapping and its potential role in gene regulation.

Main Methods:

  • Utilized Pacific Biosciences RS II long-read and Illumina HiScanSQ short-read sequencing technologies.
  • Performed in-depth bioinformatic analysis of sequencing data.
  • Focused on accurate determination of 5' and 3' transcript ends.

Main Results:

  • Identified nine novel RNA molecules, effectively doubling the known PCV-1 transcriptome.
  • Discovered a novel 3'-UTR length isoform of the Cap transcript and a non-spliced Cap transcript variant.
  • Revealed a 3'-UTR isoform of Rep, two 5'-UTR isoforms of Rep' transcripts, a novel splice variant of Rep', and a previously undetected mRNA of ORF3.
  • Observed intricate patterns of transcript overlapping, suggesting potential transcriptional interference.

Conclusions:

  • The study significantly expands the known repertoire of PCV-1 transcripts.
  • The identified novel isoforms and variants provide new insights into PCV-1 gene expression.
  • Transcriptional interference due to overlapping transcripts may represent a novel mechanism for gene regulation in circoviruses.