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Related Experiment Video

Updated: Jul 5, 2026

Preparation of Viral DNA from Nucleocapsids

Preparation of Viral DNA from Nucleocapsids

Published on: August 16, 2011

Veterinary Herpesviruses: Experimental Tools for Transcriptomics and Neuroscience.

Zsolt Boldogkői¹, Gábor Torma¹, Dóra Tombácz¹

¹Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Somogyi u. 4., 6720 Szeged, Hungary.

Veterinary Sciences

|March 27, 2026

Summary

This summary is machine-generated.

Long-read RNA sequencing of veterinary alphaherpesviruses revealed complex transcriptomes. These viral models advanced understanding of transcriptional overlaps, isoforms, and non-coding RNAs, improving genomic annotation methods.

Keywords:

RNA isoforms alphaherpesvirus gammaherpesvirus long-read sequencing nanopore sequencing non-coding RNAs replication origin transcriptional interference transcriptomics transneuronal tract-tracing veterinary herpesvirus

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Last Updated: Jul 5, 2026

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Area of Science:

Genomics
Virology
Transcriptomics

Background:

Veterinary alphaherpesviruses (pseudorabies virus, equid alphaherpesvirus 1, bovine alphaherpesvirus 1) are key models for studying viral transcriptomes.
Short-read RNA sequencing has limitations in resolving complex transcriptomic features.

Purpose of the Study:

To investigate transcriptome complexity using long-read RNA sequencing in veterinary alphaherpesviruses.
To characterize major transcriptomic features like overlaps, isoforms, and non-coding RNAs.
To establish these viral datasets as benchmarks for genomic analysis tools.

Main Methods:

Long-read RNA sequencing (viromics) of three veterinary alphaherpesviruses.
Analysis of transcriptional overlaps, alternative splicing, and transcript termini.
Evaluation of transcript callers and annotation pipelines using viral data.

Main Results:

Discovery of widespread transcriptional overlaps complicating read assignment and potentially driving interference.
Identification of diverse transcript isoforms due to alternative 5'/3' termini and splicing.
Characterization of non-coding RNAs near replication origins, suggesting replication-transcription interactions.
Demonstration of veterinary herpesvirus datasets as stringent benchmarks for genomic analysis tools, improving isoform calling and mapping.

Conclusions:

Long-read viromics in veterinary systems significantly enhanced understanding of transcriptome complexity.
These viral models have been crucial in developing best practices for full-length transcript analysis and annotation.
Veterinary gammaherpesviruses serve as valuable proxies for studying human gammaherpesviruses, and pseudorabies virus has applications in neurotracing.