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Long-read Transcriptomics of Caviid Gammaherpesvirus 1: Compiling a Comprehensive RNA Atlas.

Gábor Torma1, Ákos Dörmő1, Ádám Fülöp1

  • 1Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary.

Biorxiv : the Preprint Server for Biology
|December 23, 2024
PubMed
Summary
This summary is machine-generated.

Caviid gammaherpesvirus 1 (CaGHV-1) transcriptome was mapped using nanopore sequencing. This oncogenic virus offers a new model for studying human gammaherpesvirus pathogenesis.

Keywords:
Caviid gammaherpesvirus 1 (CaGHV-1)direct RNA sequencinggammaherpesvirusguinea pig herpes-like viruslong-read sequencingluciferase reporter assaynanopore sequencingtranscriptome

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Area of Science:

  • Virology
  • Genomics
  • Transcriptomics

Background:

  • Caviid gammaherpesvirus 1 (CaGHV-1), a guinea pig herpes-like virus, is oncogenic but its transcriptome remains uncharacterized.
  • Understanding CaGHV-1's gene expression is crucial for its use as a research model.

Purpose of the Study:

  • To annotate the CaGHV-1 genome and create a detailed transcriptomic atlas.
  • To investigate the transcriptional regulation mechanisms of CaGHV-1.

Main Methods:

  • Nanopore long-read RNA sequencing was employed for genome-wide transcript analysis.
  • Promoter elements and transcriptional overlap were mapped and analyzed.

Main Results:

  • A comprehensive transcriptomic atlas of CaGHV-1 was constructed, revealing diverse viral mRNAs and non-coding RNAs.
  • The CaGHV-1 RTA transcription factor was shown to activate its own promoter, and its ORF50 promoter responded to heterologous RTA proteins.
  • Extensive transcriptional overlap was identified, suggesting a role in global gene expression regulation.

Conclusions:

  • This study provides the first detailed transcriptomic map of CaGHV-1.
  • CaGHV-1 exhibits conserved RTA-mediated transcriptional mechanisms with other gammaherpesviruses.
  • CaGHV-1 is established as a valuable small animal model for human gammaherpesvirus pathogenesis research due to its tumorigenic properties and host range.