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Updated: Mar 1, 2026

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
Published on: September 11, 2020
Co-elution effects can influence molar mass determination of large macromolecules with asymmetric flow field-flow
Daysi Perez-Rea1, Claudia Zielke2, Lars Nilsson2
1Center for Food and Natural Products, San Simon University, Cochabamba, Bolivia; Food Colloids Group, Department of Food Technology, Engineering and Nutrition, Lund University, P.O. Box 124, S-22100 Lund, Sweden.
Abstract:
Starch and hence, amylopectin is an important biomacromolecule in both the human diet as well as in technical applications. Therefore, accurate and reliable analytical methods for its characterization are needed. A suitable method for analyzing macromolecules with ultra-high molar mass, branched structure and high polydispersity is asymmetric flow field-flow fractionation (AF4) in combination with multiangle light scattering (MALS) detection. In this paper we illustrate how co-elution of low quantities of very large analytes in AF4 may cause disturbances in the MALS data which, in turn, causes an overestimation of the size. Furthermore, it is shown how pre-injection filtering of the sample can improve the results.
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