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Updated: Mar 1, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Structural studies of the O-specific polysaccharide from detergent degrading bacteria Pseudomonas putida TSh-18
Elena N Sigida1, Yuliya P Fedonenko1, Alexander S Shashkov2
1Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049 Saratov, Russia.
Abstract:
An O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of bacteria Pseudomonas putida TSh-18, capable of degrading non-ionogenic technical detergents. The polysaccharide was found to contain a rarely occurring sugar derivative 4,6-dideoxy-4-[(R)-3-hydroxybutanoylamino]-d-galactose [d-Fucp4N(RHb)]. Sugar and methylation analyses, Smith degradation, solvolysis with CF3CO2H, and 1H and 13C NMR spectroscopy enabled elucidation of the following structure of the branched trisaccharide repeating units of the polysaccharide.
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