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Ginsenoside Rb1 exerts antidiabetic action on C2C12 muscle cells by leptin receptor signaling pathway

Mohammad Reza Tabandeh1, Seyed Ahmad Hosseini2, Maryam Hosseini1

  • 1a Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine , Shahid Chamran University of Ahvaz , Ahvaz , Iran.

Journal of Receptor and Signal Transduction Research
|May 31, 2017
PubMed
Abstract

Insights

Ginsenoside Rb1 enhances glucose uptake in muscle cells by increasing leptin receptors and activating the PI3K pathway. This study clarifies Rb1

Area of Science:

  • * Molecular biology
  • * Cellular metabolism
  • * Endocrinology

Background:

  • * Ginsenoside Rb1 (Rb1) is known to improve insulin sensitivity and glucose uptake in muscle cells.
  • * The specific effect of Rb1 on leptin signaling in skeletal muscle remains unclear.
  • * Leptin signaling plays a crucial role in regulating energy balance and glucose metabolism.

Purpose of the Study:

  • * To investigate the impact of Rb1 on leptin receptors (ObR) expression in C2C12 skeletal muscle cells.
  • * To elucidate the role of Rb1 in activating key leptin signaling pathways, including STAT3, PI3K, and ERK.
  • * To determine the mechanism by which Rb1 influences glucose transporter 4 (GLUT4) translocation.

Main Methods:

  • * C2C12 myotubes were treated with varying concentrations of Rb1 (0.1–10 μM) for 1–12 hours.
  • * Leptin receptor expression and GLUT4 translocation were quantified using real-time PCR and Western blot.
  • * Specific inhibitors of PI3K (wortmannin) and ERK (PD98059) were used to dissect signaling pathways.

Main Results:

  • * Rb1 (10 μM, 3 hours) significantly upregulated ObRa and ObRb mRNA and protein expression.
  • * Rb1 stimulated phosphorylation of STAT3, PI3K, and ERK2.
  • * Rb1-induced GLUT4 translocation was dependent on ObRb and primarily mediated by PI3K activation, not ERK.

Conclusions:

  • * Ginsenoside Rb1 promotes GLUT4 translocation in skeletal muscle cells.
  • * This effect is achieved through the upregulation of leptin receptors and subsequent activation of the PI3K signaling pathway.
  • * Rb1's action on glucose uptake is linked to enhanced leptin receptor signaling.
Keywords:
C2C12 myotubeGLUT4 translocationGinsenoside Rb1leptin receptors

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