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Updated: Mar 1, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
Published on: July 13, 2012
A pilot-scale study on the start-up of partial nitrification-anammox process for anaerobic sludge digester liquor
Gang Wang1, Xiaochen Xu1, Liang Zhou1
1Key Laboratory of Industrial Ecology and Environmental Engineering (China Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
Abstract:
Treatment of sludge digester liquor was successfully accomplished using a pilot-scale partial nitrification-anammox (PN/A) reactor with a nitrogen removal rate (NRR) of 1.23kgN/m3/d. A stable and efficient PN process was attained by controlling the concentration of free ammonia (0.7-8.4mg/L) and free nitrous acid (0.02-1.0mg/L). The application of hydroxylamine played a vital role in the reactivation of anammox bacteria. The bacteria exhibited improved granule properties at a specific input power between 0.065 and 0.097kW/m3, and achieved a specific anammox activity (SAA) of 1.01kgN/kgVSS/d on day 148. From day 0 to 120, the heme c content in the granules increased from 0.42±0.1 to 5.77±1.0µmol/gVSS, with a corresponding increase in NRRs and SAAs. High-throughput sequencing techniques revealed that the dominant anammox bacterial genus was Candidatus Brocadia. These conclusions provide valuable information for the full-scale treatment of sludge digester liquor.

