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Long Non-Coding RNA Expression Profiling in Obesity Mice with Folic Acid Supplement
Feifei Ma1, Wei Li2, Renqiao Tang2
1Department of Biochemistry, Capital Institute of Pediatrics, Beijing, China.
Summary
Folic acid (FA) may improve cardiovascular function in obesity by regulating lncRNAs and mRNAs involved in inflammation and energy metabolism. This study identified potential biomarkers for high-fat diet-related obesity.
Area of Science:
- Cardiovascular Biology
- Molecular Biology
- Nutritional Science
Background:
- Obesity is a significant risk factor for metabolic and cardiovascular diseases.
- Understanding molecular mechanisms underlying obesity-related cardiovascular dysfunction is crucial.
Purpose of the Study:
- To investigate the effect of folic acid (FA) on the genome-wide expression of lncRNAs and mRNAs in the heart of obese mice.
- To identify potential molecular targets for managing obesity-related cardiovascular issues.
Main Methods:
- Comparative analysis of lncRNA and mRNA expression profiles in hearts from mice on standard, high-fat, and high-fat with FA diets.
- Validation of differentially expressed genes using quantitative reverse transcription polymerase chain reaction (qRT-PCR).
- Bioinformatics and co-expression analyses to predict functions and regulatory relationships.
Main Results:
- Microarray analysis revealed differential expression of 58,952 lncRNAs and 20,145 mRNAs between high-fat diet and FA-supplemented groups.
- Gene Ontology and pathway analyses linked differentially expressed genes to inflammation, energy metabolism, and cell differentiation.
- Three lncRNAs (NONMMUT033847, NONMMUT070811, NONMMUT015327) and several mRNAs showed significant expression changes, potentially regulating key biological processes.
Conclusions:
- Folic acid supplementation may ameliorate cardiovascular dysfunction in obesity.
- Identified lncRNAs and mRNAs associated with inflammation and cell differentiation could serve as potential biomarkers or therapeutic targets for obesity-related cardiovascular disease.
