Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

16.8K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
16.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The critical role of the endogenous immune compartment after CAR T cell therapy in recurrent GBM.

Cell·2026
Same author

Transformer-based Fusion of Longitudinal Multimodal Radiomic Features from Chest Radiography and CT in COVID-19.

Radiology. Artificial intelligence·2026
Same author

Development of microRNA-Based Glioblastoma Biomarkers Using Blood Plasma Specimens.

Diagnostics (Basel, Switzerland)·2026
Same author

Improving a Plasma Biomarker Panel for Early Detection of Pancreatic Ductal Adenocarcinoma with Aminopeptidase N (ANPEP) and Polymeric Immunoglobulin Receptor (PIGR).

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Plasma cell-free DNA markers predict occult metastases in patients with resectable pancreatic ductal adenocarcinoma.

Clinical and translational medicine·2026
Same author

Development of a prognostic multiomic biomarker of progression-free survival in advanced non-small cell lung cancer patients treated with first line immunotherapy.

Scientific reports·2025

Related Experiment Video

Updated: Mar 1, 2026

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
05:17

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

9.2K

Flow Cytometric Methods for Circulating Tumor Cell Isolation and Molecular Analysis.

Neha Bhagwat1, Erica L Carpenter2

  • 1Division of Gastroenterology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Advances in Experimental Medicine and Biology
|June 1, 2017
PubMed
Summary

Circulating tumor cells (CTCs) offer a non-invasive method for cancer detection and monitoring. Flow cytometry enables efficient isolation and analysis of these rare cells for research and diagnostics.

Keywords:
Acoustic focusingCell sortingCirculating tumor cellsFlow cytometry

More Related Videos

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
07:32

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Published on: June 15, 2012

27.3K
Capture and Release of Viable Circulating Tumor Cells from Blood
08:10

Capture and Release of Viable Circulating Tumor Cells from Blood

Published on: October 28, 2016

9.0K

Related Experiment Videos

Last Updated: Mar 1, 2026

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
05:17

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

9.2K
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
07:32

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Published on: June 15, 2012

27.3K
Capture and Release of Viable Circulating Tumor Cells from Blood
08:10

Capture and Release of Viable Circulating Tumor Cells from Blood

Published on: October 28, 2016

9.0K

Area of Science:

  • Oncology
  • Cell Biology
  • Biotechnology

Background:

  • Circulating tumor cells (CTCs) are a valuable, non-invasive source of tumor material for cancer management.
  • CTCs aid in screening, diagnosis, therapy monitoring, target identification, and resistance assessment.
  • Novel technologies are emerging for detecting and analyzing rare CTCs in cancer patients.

Purpose of the Study:

  • To highlight the utility of circulating tumor cells in cancer management.
  • To discuss the role of flow cytometry in CTC detection and analysis.

Main Methods:

  • Utilizing flow cytometry for high-throughput and versatile analysis of rare cells.
  • Employing commercially available antibodies and fluorescent probes for cell characterization.
  • Recovering viable, high-purity cells for downstream molecular analysis.

Main Results:

  • Flow cytometry is increasingly adopted for CTC analysis due to its capabilities.
  • The technology allows for detailed phenotypic and genotypic characterization of CTCs.
  • Viable cell recovery facilitates subsequent molecular investigations.

Conclusions:

  • Circulating tumor cells are crucial for comprehensive cancer management.
  • Flow cytometry is an attractive technology for both cancer research and diagnostic applications.
  • The ability to isolate pure, viable CTCs supports advanced molecular studies.