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Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
Published on: February 3, 2013
THE EFFECTS OF KIN-STRUCTURED COLONIZATION ON NUCLEAR AND CYTOPLASMIC GENETIC DIVERSITY
Michael J Wade1, Mark L McKnight2, H Bradley Shaffer2
1Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, 60637.
Abstract:
We investigate kin-structured migration and its effects on patterns of genetic variation in cytoplasmic and nuclear genomes. We show that colonization events involving close relatives, such as those that might characterize range expansion and the invasion of new habitats, can change the patterns of nuclear and cytoplasmic genetic variation expected at equilibrium. The difference in the patterns of variation between the nuclear and mitochondrial genomes suggests that it may be possible to estimate the time of the effective kin-structured colonization event. Observations in several taxa are discussed in light of these findings and in relation to their known geological history.
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