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LOW FREQUENCY OF t HAPLOTYPES IN NATURAL POPULATIONS OF HOUSE MICE (MUS MUSCULUS DOMESTICUS)
Kristin G Ardlie1, Lee M Silver1
1Department of Ecology and Evolutionary Biology and Department of Molecular Biology, Princeton University, Princeton, New Jersey, 08544.
T haplotypes, a meiotic-drive system in mice, are rarer than expected in wild populations. Their frequency varies with population size and stability, being lower in large, stable populations and higher in small, unstable ones.
Area of Science:
- Genetics
- Evolutionary Biology
- Population Dynamics
Background:
- T haplotypes are autosomal meiotic-drive systems on mouse chromosome 17.
- They exhibit non-Mendelian transmission, favoring inheritance from heterozygous males.
- Expected rapid fixation contrasts with typically low observed frequencies in natural populations.
Purpose of the Study:
- To investigate the frequency and distribution of t haplotypes in wild house mice (Mus musculus domesticus).
- To utilize DNA markers for large-scale genotyping, overcoming limitations of previous breeding-based methods.
- To analyze the relationship between t haplotype frequency and population characteristics.
Main Methods:
- Large-scale survey of wild mice (Mus musculus domesticus).
- Genotyping using DNA markers to identify t haplotype carriers.
- Analysis of t haplotype frequencies in relation to population size and stability.
Main Results:
- Overall frequency of +/t heterozygotes was 0.062, significantly lower than prior estimates.
- T haplotypes were patchily distributed and rare, found in only 46% of sampled populations.
- Frequencies were lower in large, stable populations and higher, more variable in small, unstable populations.
Conclusions:
- T haplotype frequencies in wild mouse populations are lower than previously reported.
- Population size and stability are key factors influencing t haplotype distribution.
- Small, unstable populations may serve as crucial reservoirs for t haplotypes.
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