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Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry.
J S McGrath1, C Honrado2, D Spencer2
1Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, EH14 4AS, United Kingdom.
Scientific Reports
|June 3, 2017
Summary
Microfluidic Impedance Cytometry rapidly identifies and analyzes the viability of single protozoan pathogens like Cryptosporidium and Giardia. This technology offers faster, more accurate detection of these parasites compared to current methods.
Area of Science:
- Parasitology
- Biotechnology
- Analytical Chemistry
Background:
- Limited technologies exist for single-(oo)cyst level detection and viability analysis of protozoan pathogens.
- Accurate identification of Cryptosporidium and Giardia is crucial for public health.
Purpose of the Study:
- To utilize Microfluidic Impedance Cytometry (MIC) for characterizing AC electrical properties of single parasites.
- To demonstrate rapid discrimination of protozoan pathogens based on viability and species.
Main Methods:
- Microfluidic Impedance Cytometry (MIC) was employed to measure the impedance of individual parasite (oo)cysts.
- AC electrical properties were analyzed to differentiate between live, inactive, and damaged/excysted oocysts.
Main Results:
- MIC achieved over 90% certainty in identifying live and inactive Cryptosporidium parvum oocysts.
- Discrimination between Cryptosporidium parvum, Cryptosporidium muris, and Giardia lamblia reached over 92% certainty.
- Enumeration and identification of (oo)cysts were accomplished within minutes.
Conclusions:
- MIC provides a rapid and accurate method for the detection, identification, and viability assessment of protozoan pathogens at the single (oo)cyst level.
- This technology significantly reduces identification time and labor compared to existing methods.
- MIC shows promise for improved diagnostics and monitoring of Cryptosporidium and Giardia infections.
