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Recent Advances in Antibody Discovery Using Ultrahigh-Throughput Droplet Microfluidics: Challenges and Future
Dhiman Das1, John Scott McGrath1, John Hudson Moore1
1Ampersand Biomedicines Inc., Southline Suite 3A, 135 William T. Morrissey Blvd., Boston, MA 02125, USA.
Biosensors
|July 25, 2025
Summary
Droplet microfluidics significantly accelerates antibody discovery by enabling precise control of picoliter droplets for high-throughput screening. This review highlights technological advances and methods for efficient antibody identification.
Area of Science:
- Biotechnology
- Microfluidics
- Immunology
Background:
- Droplet microfluidics offers high-throughput antibody discovery.
- It enables precise control and manipulation of picoliter-volume droplets.
Purpose of the Study:
- To provide a comprehensive overview of recent advances in droplet microfluidics for antibody discovery.
- To focus on technological and methodological innovations enhancing the antibody discovery process.
Main Methods:
- Review of fundamental principles of droplet microfluidics.
- Discussion of various assay types: binding, functional, FRET, internalization, and neutralization assays.
- Examination of methods for droplet encapsulation and challenges like small molecule leakage.
Main Results:
- Assessment of design considerations for high-throughput fluorescence-activated droplet sorting (FADS) workstations.
- Emphasis on the importance of automation in droplet microfluidics.
- Analysis of the commercial landscape and emerging industry trends.
Conclusions:
- Droplet microfluidics is a transformative technology for antibody discovery.
- Further research and innovation are needed for more efficient and robust antibody identification solutions.
