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Updated: Mar 1, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
Effects of starch concentration on calcium-enhanced black bullhead catfish protein gels
Ilgin Paker1,2, Kristen E Matak2
1Food Engineering Department Yeditepe University Kayisdagi Istanbul Turkey.
Abstract:
Calcium-enhanced protein recovered from black bullhead catfish was used to develop gels containing increasing amounts of potato starch (0-20 g/kg protein paste) and the effects of starch on functional, textural, and color properties were tested. Energy required to unfold protein groups was greater with the addition of 5 g starch/kg protein paste. Gels containing starch were harder, chewier, and less springy (p < .05) than gels without starch. For most measurements, regression analysis showed that increasing the starch concentration beyond 5 g/kg did not contribute to further significant textural changes. Torsional shear stress and strain along with Kramer shear force increased as the concentration of starch increased (R = .79, .79, and .53, respectively). The addition of ≥10 g starch/kg protein paste resulted in darker gels and gels got darker as more starch was added (R = .71). Results showed no benefit to increasing starch concentration in gels beyond 5 g starch/kg protein paste.
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